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Turkish Journal of Chemistry

Abstract

Terbutaline hemisulfate (THS), a selective beta-2 agonist, is preferred as a bronchodilator for the treatment of asthma and bronchitis, as well as a tocolytic agent to delay labor in acute situations during premature birth. The use of beta-2 agonists above a certain dose is considered doping in sports competitions. Therefore, it is important that they be determined with high sensitivity and selectivity within a short analysis time. In this study, a SnO2 nanoparticle-modified carbon paste electrode was developed for the determination of THS. Cyclic voltammetry and differential pulse voltammetry were employed as electrochemical methods. The operating conditions of the developed SnO2 nanoparticle-modified carbon paste electrode (SnO2NP/CPE), including the scan rate, SnO2 nanoparticle content, and pH, were optimized. At the end of the experiments, the optimum operating conditions were determined to be a scan rate of 10 mV/s, 5% SnO2 nanoparticles, and 0.25 M acetate buffer at pH 4.0. The analytical parameters of SnO2NP/CPE were also determined. The linear range of the nanosensor was 2.0–50.0 μM, with a limit of detection of 0.55 μM and a limit of quantification of 1.8 μM. In the reproducibility and repeatability tests, the coefficients of variation for 30 μM THS, based on 10 measurements, were 2.58% and 3.30%, respectively. No significant interference from NaCl, glucose, urea, and uric acid was observed in the interference experiments. In contrast, ascorbic acid, dopamine, epinephrine, and citric acid showed slight interference. Comparing the sensor response obtained on the 14th day with the initial peak current value of the nanosensor revealed no change in the nanosensor response, indicating that the developed nanosensor has good storage stability. The developed nanosensor (SnO2NP/CPE) can be prepared easily and used directly for the determination of THS in samples without additional separation or extraction steps. It is considered suitable for the determination of THS because of its advantages, including good reproducibility and reliability, low cost, and a short response time.

Author ORCID Identifier

RAUF GULAMOV: 0009-0007-7222-2701

ŞENOL ALPAT: 0000-0001-5937-9949

SİBEL KILINÇ ALPAT: 0000-0001-7149-3779

DOI

10.55730/1300-0527.3907

Keywords

Nanosensor, terbutaline hemisulfate, beta-2 agonists, differential pulse voltammetry, cyclic voltammetry

First Page

423

Last Page

437

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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