Turkish Journal of Chemistry
Abstract
A label-less impedimetric biosensor modified with poly(thiophene)-graft-poly(glycidyl methacrylate) polymer (PThi-g-PGM) was manufactured for p53 protein quantification in human serum samples. In this study, PThi-g-PGM polymer was synthesized, and this polymer matrix was coated using the spin-coating technique on the single-use indium tin oxide (ITO) substrate for anti-p53 antibody immobilization. The anti-p53 antibodies with high affinity for p53 proteins were covalently attached onto the PThi-g-PGM-coated electrode surface. In addition, the affinity of anti-p53 for the p53 protein was monitored at a constant frequency. Under optimized conditions, the impedimetric changes were linearly related to the p53 concentrations, ranging from 0.05 to 15 pg/mL with a low detection limit of 15.9 fg/mL. This biosensor had desirable storage stability, acceptable repeatability, and high reproducibility. Moreover, this impedimetric biosensor could be regenerated through an acidic treatment procedure. Additionally, the suggested biosensor successfully detected the p53 antigen in human serum samples, and good recycling rates (98.41%–109.32%) were found. In summary, the proposed immunosensor may be a powerful tool for the analysis of the p53 protein for early detection of cancer biomarkers.
Author ORCID Identifier
MUHAMMET AYDIN: 0000-0002-8716-9970
DOI
10.55730/1300-0527.3736
Keywords
Graft polymer, p53, impedimetric biosensor, electrochemical analysis
First Page
371
Last Page
381
Publisher
The Scientific and Technological Research Council of Türkiye (TÜBİTAK)
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
AYDIN, M (2025). Determination of p53 biomarker with a smart electrochemical biosensor based on brush polymer-functionalized disposable electrode. Turkish Journal of Chemistry 49 (3): 371-381. https://doi.org/10.55730/1300-0527.3736
