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Turkish Journal of Chemistry

Abstract

Sex-Determining Region Y-box 2 (SOX2) is a transcription factor protein. SOX2 expression is related to lymph nodemetastasis and distant metastasis in colorectal carcinomas. SOX2 was determined with the first magnetic disposable immunoplatform.The designed sandwich-shaped immune complexes were formed by a capture antibody, SOX2 protein, and biotinylated secondaryantibodies (dAb/HRP). The sandwich-shaped immune complex was linked to carboxylic acid functionalized magnetic beads (HOOC-MBs). This magnetic bioconjugate was dropped on the surface of the screen-printed carbon electrode (SPCE). The amperometricmeasurement was performed at –0.20 V in the presence of hydroquinone (HQ) and H2O2 against a silver pseudo-reference electrode.The optimization parameters affecting the immunoassay response were evaluated.The analytical evaluation of the magnetic disposable immunoplatform for the amperometric detection of SOX2 standards was done.The developed immunosensor shows high sensitivity (LOD of 1.37 ng mL−1) and a short analysis time (15 min). Potential interferingcompounds found in serum samples were tested. The storage stability of magnetic disposable immunoplatform was evaluated. Thedeveloped immunosensor was compared with the ELISA method.

Author ORCID Identifier

GÖKSU ÖZÇELİKAY AKYILDIZ: 0000-0001-7919-3236

MEHMET ALTAY ÜNAL: 0000-0001-8607-5043

SEÇİL GÜLDEN: 0009-0006-7409-6705

SİBEL ÖZKAN: 0000-0001-7494-3077

DOI

10.55730/1300-0527.3712

Keywords

Amperometry, magnetic beads, immunosensor, sandwich-shaped immune complexes, SOX2 protein

First Page

79

Last Page

88

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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