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Turkish Journal of Chemistry

Author ORCID Identifier

MERVE BAT ÖZMATARA: 0000-0002-6912-8825

TUĞÇE GEVREK: 0000-0002-2297-5891

DOI

10.55730/1300-0527.3696

Abstract

Cryogels containing isocyanate reactive groups were synthesized via photopolymerization of 2-isocyanoethyl methacrylate (ICEMA) and poly(ethylene glycol) methyl ether methacrylate (PEGMEMA) monomers. Changing ratio of monomers with respect to each other, series of cryogels with varying ratio of reactive isocyanate groups were successfully prepared. Characterization of the cryogels was carried out using fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and rheometry techniques. To demonstrate that molecules containing amine groups can be immobilized onto the synthesized cryogels through the reaction between isocyanate and amine groups, cryogels were conjugated with 4-(trifluoromethyl)benzylamine (TFBA) and fluorescein amine (FLA) molecules, and the conjugations were confirmed through FTIR and fluorescence microscopy, respectively for TFBA and FLA. Additionally, FTIC-BSA immobilization was studied to illustrate that biomolecules can also be bound to the cryogels without a linker. It was shown that the amount of immobilized FTIC-labeled model proteins can be controlled by adjusting the concentration of isocyanate reactive groups within the cryogel matrix, and this functionalization was confirmed via a fluorescence microscope.

Keywords

bioimmobilization, cryogels, isocyanate-amine, reactive polymers

First Page

770

Last Page

779

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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