Turkish Journal of Chemistry
Abstract
Dye-ligand affinity chromatography is among the increasingly popular affinity chromatography based on molecular recognition for the purification of albumin. This study focuses on the binding of Cibacron Blue F3GA ligand dye with magnetic silica particles and purification by separation. Mono-disperse silica particles with bimodal pore size distribution were employed as a high-performance adsorbent for human serum albumin (HSA) protein purification under equilibrium conditions. The synthesized ligand-dye affinity based magnetic silica particles were characterized by electron spin resonance, Fourier-transform infrared spectroscopy, scanning electron microscopy, vibrating sample magnetometer, elemental analysis, and dispersive X-ray analysis. The HSA purification performance of the proposed material in the presence of a magnetic field was relatively investigated using magnetic-based particles with similar morphologies. The maximum adsorption capacity for HSA in an artificial plasma medium was defined as 48.6 mg/g magnetic silica particle. By using the designed magnetic silica particles, 1.0 M NaCl solution was successfully utilized for obtaining quantitative desorption with HSA. However, continued HSA purification performances of magnetic-based particles were significantly lower concerning the ligand-dye magnetic silica particles. The purity of the removed albumin was about 97%. The magnetic silica particles could be utilized many times without decreasing their protein adsorption capacities remarkably.
DOI
10.55730/1300-0527.3599
Keywords
Albumin purification, Cibacron blue F3GA, ligand dye, magnetic silica particles, protein purification
First Page
1125
Last Page
1137
Recommended Citation
TATAR, NURHAK; AKGÖNÜLLÜ, SEMRA; ALAGÖZ, HANDAN YAVUZ; and DENİZLİ, ADİL
(2023)
"Cibacron Blue F3GA ligand dye-based magnetic silica particles for the albumin purification,"
Turkish Journal of Chemistry: Vol. 47:
No.
5, Article 19.
https://doi.org/10.55730/1300-0527.3599
Available at:
https://journals.tubitak.gov.tr/chem/vol47/iss5/19