Determination of essential oil and biological activities of Hypericum ternatum Poulter and H. scabrum L. species collected from different localities: is H. scabrum an alternative to multifunctional species ST JOHN'S WORT (H. perforatum)?

Authors

MEHMET AKDENİZ
İSMAİL YENER
SEVGİ İRTEGÜN KANDEMİR
ŞAFAK ÖZHAN KOCAKAYA
MEHMET FIRAT
SERKAN YİĞİTKAN
NESRİN HAŞİMİ BESEN
ABDULSELAM ERTAŞ
UFUK KOLAK

DOI

10.55730/1300-0527.3494

Abstract

The importance of Hypericum L. species, being used in traditional medicine, in the scientific world is increasing day by day. Hypericum species are plants that have been used in the treatment of many diseases for a long time and have healing properties. In the current study, the essential oil compositions of Hypericum scabrum L. and H. ternatum Poulter collected from different localities in Turkey were determined by GC-MS/FID. In addition, their antioxidant, cytotoxic, and antimicrobial activities with their enzyme inhibitory potentials (cholinesterase, urease, tyrosinase, elastase, and collagenase) were investigated. Also, in vitro and in silico studies of the major components of the species have been carried out on the studied enzymes. It was determined that four H. scabrum samples mainly contained α-pinene (55.99%-62.80%) while three H. ternatum samples contained 2-methyloctane (9.45%-22.39%) and α-pinene (12.75%-33.08%). While H. scabrum essential oils possessed significant biological activity potential except for the antimicrobial activity, H. ternatum samples did not. All essential oil samples of H. scabrum exhibited a high cytotoxic effect (IC₅₀ 21.67 ± 0.34 to 34.67 ± 0 .45 μg/mL) against colon cancer cell line (HT-29) and indicated higher acetyl- (Inhibiton% 83.30 ± 1.90 to 93.08 ± 1.04) and butyrylcholinesterase (Inhibiton% 80.58 ± 1.19 to 93.28 ± 1.99) inhibitory activity than the standard compound, galantamine. Furthermore, all samples of H. scabrum showed high tyrosinase (Inhibiton% 69.00 ± 1.64 to 95.25 ± 1.42) and elastase (Inhibiton% 27.58 ± 0.34 to 36.69 ± 0.18) inhibitory effects. These biological activity results indicated that H. scabrum essential oil could be used in the cosmetic and pharmaceutical industries.

Keywords

Hypericum ternatum, Hypericum scabrum, essential oils, anticholinesterase, antimicrobial, molecular docking

First Page

1956

Last Page

1971

Recommended Citation

AKDENİZ, MEHMET; YENER, İSMAİL; KANDEMİR, SEVGİ İRTEGÜN; KOCAKAYA, ŞAFAK ÖZHAN; FIRAT, MEHMET; YİĞİTKAN, SERKAN; BESEN, NESRİN HAŞİMİ; ERTAŞ, ABDULSELAM; and KOLAK, UFUK (2022) "Determination of essential oil and biological activities of Hypericum ternatum Poulter and H. scabrum L. species collected from different localities: is H. scabrum an alternative to multifunctional species ST JOHN'S WORT (H. perforatum)?," Turkish Journal of Chemistry: Vol. 46: No. 6, Article 17. https://doi.org/10.55730/1300-0527.3494
Available at: https://journals.tubitak.gov.tr/chem/vol46/iss6/17