Supercritical carbon dioxide extraction of Usnea longissima (L.) Ach.: Optimization by Box-Behnken design (BBD)

Authors

CEREN ATİLA DİNÇER
CEREN GÖKALP
BENGÜ GETİREN
ATİLA YILDIZ
NURAY YILDIZ

Abstract

Usnic acid (UA) was extracted from Usnea longissima (L.) Ach. in supercritical carbon dioxide (SC-CO2) medium. The selected process parameters were extraction temperature (35-45 °C), amount of co-solvent (0%-5%) and extraction time (5-9 h). These parameters were applied to Box-Behnken design (BBD) belonging to response surface methodology (RSM) to determine optimum process parameters for the highest amount of UA in the extract. g UA/100g lichen, extraction yield % and UA content values were calculated in the range of 0.045-0.317, 2.77-5.4 and 71%-82% in different experimental conditions, respectively. The optimum conditions were predicted as 42 °C, 4.3% (ethanol) and 7.48 h. It was determined that the predicted and experimental values of g UA/100g lichen were compatible, and the suggested model was valid.

DOI

10.3906/kim-2102-66

Keywords

Usnea longissima (L.) Ach., usnic acid (UA), supercritical extraction, response surface methodology (RSM)

First Page

1248

Last Page

1256

Recommended Citation

DİNÇER, CEREN ATİLA; GÖKALP, CEREN; GETİREN, BENGÜ; YILDIZ, ATİLA; and YILDIZ, NURAY (2021) "Supercritical carbon dioxide extraction of Usnea longissima (L.) Ach.: Optimization by Box-Behnken design (BBD)," Turkish Journal of Chemistry: Vol. 45: No. 4, Article 23. https://doi.org/10.3906/kim-2102-66
Available at: https://journals.tubitak.gov.tr/chem/vol45/iss4/23