Turkish Journal of Chemistry
DOI
10.3906/kim-2004-63
Abstract
A fluorogenic probe based on a coumarin-derivative for Cu$^{2+}$ sensing in CH$_{3}$CN/H$_{2}$O media (v/v, 95/5, 5.0 \textmu M) was developed and applied in real samples. 3-(4-chlorophenyl)-6,7-dihydroxy-coumarin (MCPC) probe was obtained by synthetic methodologies and identified by spectral techniques. The probe MCPC showed remarkable changes with a ``turn-off'' fluorogenic sensing approach for the monitoring of Cu$^{2+}$ at 456 nm under an excitation wavelength of 366 nm. The response time of the probe MCPC was founded as only 1 min. The detection limit of the probe MCPC was recorded to be 1.47 nM. The binding constant and possible stoichiometric ratio (1:1) values were determined by Benesi-Hildebrand and Job's plot systems, respectively. The mechanism of the probe MCPC with Cu$^{2+}$ was further confirmed by ESI-MS and FT-IR analyses, as well as supported by theoretical calculations. Furthermore, the probe MCPC was successfully employed for the practical applications to sense Cu$^{2+}$ in different herbal and black tea samples. The proposed sensing method was also verified by ICP-OES method.
Keywords
Coumarin, fluorescent sensor, copper, DFT, tea samples
First Page
1148
Last Page
1163
Recommended Citation
SAVRAN, TAHİR; KARAGÖZ, ABDURRAHMAN; ELMAS, ŞÜKRİYE NİHAN KARUK; AYDIN, DUYGU; ÖZEN, FURKAN; KORAN, KENAN; ARSLAN, FATMA NUR; GÖRGÜLÜ, AHMET ORHAN; and YILMAZ, İBRAHİM
(2020)
"Fluorescent sensing platform for low-cost detection of Cu$^{\mathbf{2+}}$ by coumarin derivative: DFT calculation and practical application in herbal and black tea samples,"
Turkish Journal of Chemistry: Vol. 44:
No.
4, Article 21.
https://doi.org/10.3906/kim-2004-63
Available at:
https://journals.tubitak.gov.tr/chem/vol44/iss4/21
