Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1

Authors

BERFİN VURAL
MELTEM ÇALIŞKAN
MUSTAFA KEMAL SEZGİNTÜRK

Abstract

The aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH$_{4}$ OH/H$_{2}$O$_{2}$/H$_{2}$O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS) was used. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the immobilization steps. A variety of parameters, 3-GOPS and anti-TRAP1 concentrations, and anti-TRAP1 and TRAP1 incubation durations were optimized. After determining the optimum conditions, characterization studies such asrepeatability,reproducibility,regeneration,squarewavevoltammetry,andsinglefrequencyimpedancewereperformed. The electrochemical immunosensor has presented an extremely wide determination range for TRAP1 from 0.1 pg/mL to 100 pg/mL.

DOI

10.3906/kim-1909-53

Keywords

3-GOPS, ITO-PET, TRAP1, immunosensor, biosensor

First Page

461

Last Page

471

Recommended Citation

VURAL, BERFİN; ÇALIŞKAN, MELTEM; and SEZGİNTÜRK, MUSTAFA KEMAL (2020) "Development of a biosensor platform based on ITO sheets modified with 3-glycidoxypropyltrimethoxysilane for early detection of TRAP1," Turkish Journal of Chemistry: Vol. 44: No. 2, Article 15. https://doi.org/10.3906/kim-1909-53
Available at: https://journals.tubitak.gov.tr/chem/vol44/iss2/15