Determination of triacylglycerols and fatty acid composition of Chilopsis linearis seed oil by reverse-phase high-performance liquid chromatography

Authors

ANH VAN NGUYEN
VICTOR IVANOVIC DEINEKA
LYUDMILA ALEKSANDROVNA DEINEKA
ANH THI NGOC VU

DOI

10.3906/kim-1901-26

Abstract

In the present work, the compositions of the 27 triacylglycerols (TAGs) as well as fatty acid composition of Chilopsis linearis seed oil were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) with diode array and mass spectrometric detections. It was shown that the main conjugated acid in the seed oil is a catalpic acid, (9E,11E,13Z)-octadeca-9,11,13-trienoic acid, while two unusual fatty acids have the parameters satisfying the literature data for (10E,12E)-octadeca-10,12-dienoic and (9E,12E)-octadeca-9,12-dienoic acids. For the two conjugated acid substituents the isosbestic point (at 234 nm) was found and was used to calculate the ratios of TAGs with different chromophores in the oil, taking into account the peak areas on the oil chromatogram.

Keywords

Seed oil, Chilopsis linearis, triacylglycerols, conjugated fatty acid

First Page

881

Last Page

889

Recommended Citation

NGUYEN, ANH VAN; DEINEKA, VICTOR IVANOVIC; DEINEKA, LYUDMILA ALEKSANDROVNA; and VU, ANH THI NGOC (2019) "Determination of triacylglycerols and fatty acid composition of Chilopsis linearis seed oil by reverse-phase high-performance liquid chromatography," Turkish Journal of Chemistry: Vol. 43: No. 3, Article 13. https://doi.org/10.3906/kim-1901-26
Available at: https://journals.tubitak.gov.tr/chem/vol43/iss3/13