Turkish Journal of Chemistry
Abstract
A novel "turn off-on" sensor for detection of interaction between DNA and acrylamide (ACR) was developed. In this method, L-cysteine capped Mn-doped ZnS quantum dots (QDs) were used as room temperature phosphorescent probes. In the ``turn-off'' mode, ACR was absorbed onto the surface of QDs via electrostatic interaction, which caused a quenching effect of room-temperature phosphorescence signal by photoinduced electron-transfer mechanism. ACR was removed from the QDs' surface with the addition of DNA. Thus, the phosphorescence emission of QDs was recovered and the system was turned to the ``turn-on'' mode. The quenching mechanism of QDs by ACR was collisional (dynamic) and the quenching constant, binding constant, and binding site number were calculated as 3.2 x 10$^{4\, }$ M$^{-1}$, 2.04 x 10$^{4\, }$ M$^{-1}$, and 1.2, respectively. An absorption spectrometric method was also used to evaluate ACR-DNA interaction and the binding constant (K) was found as 2.4 x 10$^{5\, }$ M$^{-1}$. The developed biosensor is simple, is free of interferences coming from autofluorescence and scattering light, and does not need any derivatization step or sample pretreatment.
DOI
10.3906/kim-1807-59
Keywords
Acrylamide, DNA interaction, quantum dots, phosphorescence, photoinduced electron transfer
First Page
125
Last Page
136
Recommended Citation
KARA, HAYRİYE EDA ŞATANA
(2019)
"Novel "turn off-on" sensors for detection of DNA-acrylamide interaction using ZnS quantum dots as a phosphorescent probe,"
Turkish Journal of Chemistry: Vol. 43:
No.
1, Article 11.
https://doi.org/10.3906/kim-1807-59
Available at:
https://journals.tubitak.gov.tr/chem/vol43/iss1/11
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