Spectroscopic and thermodynamic approach to the interaction of nonperipherally substituted cationic phthalocyanines with calf thymus (CT)-DNA

Authors

SEVGİCAN TUNCER
İBRAHİM ÖZÇEŞMECİ
BEHİCE ŞEBNEM SESALAN
AYFER KALKAN BURAT

DOI

10.3906/kim-1705-59

Abstract

Novel, nonperipherally tetrasubstituted cationic metal-free and metallophthalocyanines (Zn, In) were synthesized in the present study. The binding constants, the disappearance of quenching effect of all cationic phthalocyanines on the fluorescence intensity of SYBR Green--disodium salt of deoxyribonucleic acid (DNA) from calf thymus complex, and the changes in T$_{m}$ of double helix DNA with thermal denaturation profile were investigated by UV-Vis and fluorescence spectrophotometric methods. To investigate the spontaneity of the reactions between DNA and novel quaternized phthalocyanines in buffer, thermodynamic parameters were employed.

Keywords

Calf thymus (CT)-DNA, morpholine, quaternized phthalocyanines, Gibbs, entropy

First Page

274

Last Page

290

Recommended Citation

TUNCER, SEVGİCAN; ÖZÇEŞMECİ, İBRAHİM; SESALAN, BEHİCE ŞEBNEM; and BURAT, AYFER KALKAN (2018) "Spectroscopic and thermodynamic approach to the interaction of nonperipherally substituted cationic phthalocyanines with calf thymus (CT)-DNA," Turkish Journal of Chemistry: Vol. 42: No. 2, Article 7. https://doi.org/10.3906/kim-1705-59
Available at: https://journals.tubitak.gov.tr/chem/vol42/iss2/7