Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes

Authors

HÜSEYİN İSTANBULLU
HAKAN KARADENİZ
ERÇİN ERCİYAS
KADRİYE ARZUM ERDEM GÜRSAN

Abstract

Four different Mannich base derivatives containing an aromatic/heteroaromatic propanone structure (C1 (3-(dimethylamino)-1-(thiophen-2-yl)propan-1-one hydrochloride); C2 (3-morpholino-1-(pyridin-3-yl)propan-1-one hydrochloride); C3 (3-(dimethylamino)-1-(1H-indol-3-yl)propan-1-one hydrochloride), C4 (3-(piperidin-1-yl)-1-(pyren-1-yl)propan-1-one hydrochloride)) were earlier synthesized and characterized with $^{1}$H NMR, $^{13}$C NMR, MS, and elemental analysis. The interaction of these compounds with fish sperm double stranded DNA (fs-dsDNA) was investigated by using differential pulse voltammetry (DPV) in connection with a disposable pencil graphite electrode (PGE). After the interaction procedure, there was a meaningful decrease in the oxidation signal of the electroactive DNA base guanine due to possible intercalation and/or alkylation mechanism between these Mannich base derivatives and DNA. The features of this electrochemical assay were discussed in comparison to previous reports related to DNA targeted agents/drug candidates in the literature.

DOI

10.3906/kim-1601-1

Keywords

Electrochemical DNA biosensor, Mannich base, pencil graphite electrode, differential pulse voltammetry

First Page

40

Last Page

47

Recommended Citation

İSTANBULLU, HÜSEYİN; KARADENİZ, HAKAN; ERCİYAS, ERÇİN; and GÜRSAN, KADRİYE ARZUM ERDEM (2017) "Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes," Turkish Journal of Chemistry: Vol. 41: No. 1, Article 3. https://doi.org/10.3906/kim-1601-1
Available at: https://journals.tubitak.gov.tr/chem/vol41/iss1/3