Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes

Authors

HÜSEYİN İSTANBULLU
HAKAN KARADENİZ
ERÇİN ERCİYAS
KADRİYE ARZUM ERDEM GÜRSAN

DOI

10.3906/kim-1601-1

Abstract

Four different Mannich base derivatives containing an aromatic/heteroaromatic propanone structure (C1 (3-(dimethylamino)-1-(thiophen-2-yl)propan-1-one hydrochloride); C2 (3-morpholino-1-(pyridin-3-yl)propan-1-one hydrochloride); C3 (3-(dimethylamino)-1-(1H-indol-3-yl)propan-1-one hydrochloride), C4 (3-(piperidin-1-yl)-1-(pyren-1-yl)propan-1-one hydrochloride)) were earlier synthesized and characterized with $^{1}$H NMR, $^{13}$C NMR, MS, and elemental analysis. The interaction of these compounds with fish sperm double stranded DNA (fs-dsDNA) was investigated by using differential pulse voltammetry (DPV) in connection with a disposable pencil graphite electrode (PGE). After the interaction procedure, there was a meaningful decrease in the oxidation signal of the electroactive DNA base guanine due to possible intercalation and/or alkylation mechanism between these Mannich base derivatives and DNA. The features of this electrochemical assay were discussed in comparison to previous reports related to DNA targeted agents/drug candidates in the literature.

Keywords

Electrochemical DNA biosensor, Mannich base, pencil graphite electrode, differential pulse voltammetry

First Page

40

Last Page

47

Recommended Citation

İSTANBULLU, HÜSEYİN; KARADENİZ, HAKAN; ERCİYAS, ERÇİN; and GÜRSAN, KADRİYE ARZUM ERDEM (2017) "Electrochemical detection of DNA interaction with Mannich base derivatives by disposable graphite electrodes," Turkish Journal of Chemistry: Vol. 41: No. 1, Article 3. https://doi.org/10.3906/kim-1601-1
Available at: https://journals.tubitak.gov.tr/chem/vol41/iss1/3