Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen

Authors

DIMITRIOS TSIKAS
ANKE BÖHMER
ARNE TRETTIN
ARSLAN ARINÇ KAYAÇELEBİ

DOI

10.3906/kim-1501-142

Abstract

A laboratory method for the acid-free synthesis of S-nitrosothiols (RSNO) from NaNO$_{2}$ and thiols in aqueous buffer of neutral pH is reported. Equimolar amounts of NaNO$_{2}$ and thiol are added to a 100-$\mu $M Na$_{4}$EDTA-containing Na$_{2}$HPO$_{4}$ buffer (100 mM, pH 7.4). Samples are set into liquid nitrogen for 1 min and then thawed to room temperature or in an ice bath. Dependent upon the RSNO, repetition of the procedure may be required. This method may be of particular importance when the classical acid-based method fails or releases harmful gases such as H$_{2}$S.

Keywords

LC-MS, nitric oxide, nitrite, S-nitrosothiols

First Page

676

Last Page

682

Recommended Citation

TSIKAS, DIMITRIOS; BÖHMER, ANKE; TRETTIN, ARNE; and KAYAÇELEBİ, ARSLAN ARINÇ (2015) "Acid-free synthesis of S-nitrosothiols at neutral pH by shock-freezing in liquid nitrogen," Turkish Journal of Chemistry: Vol. 39: No. 3, Article 17. https://doi.org/10.3906/kim-1501-142
Available at: https://journals.tubitak.gov.tr/chem/vol39/iss3/17