Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan--carbon nanotube composite modified electrodes

Authors

PEMBE ECE CANAVAR
ECE EKŞİN
KADRİYE ARZUM ERDEM GÜRSAN

Abstract

Single-walled carbon nanotube (CNT) and chitosan composite (chitosan*CNT) based sensors were developed as DNA biosensors, and then they were applied for electrochemical investigation of the interaction between the anticancer drug mitomycin C (MC) and DNA. The oxidation signals of MC and guanine were monitored before and after the interaction process by differential pulse voltammetry (DPV). The DPV results were in good agreement with those of electrochemical impedance spectroscopy (EIS). Analytical parameters such as DNA concentration, MC concentration, and MC interaction time with DNA were optimized. The detection limits were 6.85 \mu g/mL for DNA and 11.01 \mu g/mL for MC.

DOI

10.3906/kim-1402-11

Keywords

Chitosan, single-walled carbon nanotubes, mitomycin C, DNA interactions, disposable graphite electrode, electrochemical impedance spectroscopy, differential pulse voltammetry

First Page

1

Last Page

12

Recommended Citation

CANAVAR, PEMBE ECE; EKŞİN, ECE; and GÜRSAN, KADRİYE ARZUM ERDEM (2015) "Electrochemical monitoring of the interaction between mitomycin C and DNA at chitosan--carbon nanotube composite modified electrodes," Turkish Journal of Chemistry: Vol. 39: No. 1, Article 1. https://doi.org/10.3906/kim-1402-11
Available at: https://journals.tubitak.gov.tr/chem/vol39/iss1/1