Turkish Journal of Chemistry
DOI
10.3906/kim-1403-5
Abstract
Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO_2-esterase activity gave Ki values of 86.0 \mu M and 57.0 \mu M, respectively.
Keywords
Carbonic anhydrase I, carbonic anhydrase II, rosmarinic acid, enzyme purification, enzyme inhibition
First Page
894
Last Page
902
Recommended Citation
TOPAL, MERYEM and GÜLÇİN, İLHAMİ
(2014)
"Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor,"
Turkish Journal of Chemistry: Vol. 38:
No.
5, Article 20.
https://doi.org/10.3906/kim-1403-5
Available at:
https://journals.tubitak.gov.tr/chem/vol38/iss5/20
