In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes

Authors

BÜŞRA CEBECİ
ZUHAL ALIM
ŞÜKRÜ BEYDEMİR

DOI

10.3906/kim-1308-20

Abstract

The organophosphate target enzyme paraoxonase-1 (PON1) has been extensively studied in toxicology. Pesticides are organophosphate compounds that are commonly used in agriculture. They are also used as nerve gases, such as sarin and soman. Therefore, the effects of these compounds on enzymatic activity are well known. In the present study, we investigated the in vitro effects of fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, and dichlorvos pesticides on sheep liver microsomal PON1. The enzyme was purified approximately 141-fold with a specific activity of 1822.22 EU/mg proteins. The pesticides inhibited sheep liver PON1 in vitro, and the IC_{50} values for these compounds were 0.0103, 0.069, 0.157, and 0.2 \mu M, respectively.

Keywords

Paraoxonase, fenoxaprop-p-ethyl, lambda cyhalothrin, imidacloprid, dichlorvos, inhibition

First Page

512

Last Page

520

Recommended Citation

CEBECİ, BÜŞRA; ALIM, ZUHAL; and BEYDEMİR, ŞÜKRÜ (2014) "In vitro effects of pesticide exposure on the activity of the paraoxonase-1 enzyme from sheep liver microsomes," Turkish Journal of Chemistry: Vol. 38: No. 3, Article 16. https://doi.org/10.3906/kim-1308-20
Available at: https://journals.tubitak.gov.tr/chem/vol38/iss3/16