Establishment of an electrochemical RNA aptamer-based biosensor to trace nanomolar concentrations of codeine

Authors

MEHDI SABERIAN
DAVOUD ASGARI
YADOLLAH OMIDI
JALEH BARAR
HOSSEIN HAMZEIY

DOI

10.3906/kim-1209-45

Abstract

Codeine is an opium alkaloid with a great potential of abuse among opioid consumers. Detection of codeine is a routine procedure in the military and government of some countries for personnel recruitment. Therefore, a specific, selective, and easy to use method would be an important improvement in such detection procedures. According to previous reports, short single-stranded DNA or RNA sequences with high affinity and specificity to their targets, aptamers, could be used for designing an accurate and specific biosensor for codeine. This study introduces an aptamer-based biosensor for codeine detection in nanomolar concentrations by an electrochemical method, and ferrocene carboxylic acid was used as the redox molecule. The data show an improvement in codeine detection in comparison with the previous reports by other methods. The fabricated aptasensor offers a simpler and faster method with a lower limit of detection for codeine. The results demonstrate the reliability and robustness of the constructed aptasensor in codeine detection and measurement.

Keywords

Aptasensor, aptamer, SELEX, codeine

First Page

366

Last Page

373

Recommended Citation

SABERIAN, MEHDI; ASGARI, DAVOUD; OMIDI, YADOLLAH; BARAR, JALEH; and HAMZEIY, HOSSEIN (2013) "Establishment of an electrochemical RNA aptamer-based biosensor to trace nanomolar concentrations of codeine," Turkish Journal of Chemistry: Vol. 37: No. 3, Article 5. https://doi.org/10.3906/kim-1209-45
Available at: https://journals.tubitak.gov.tr/chem/vol37/iss3/5