Turkish Journal of Chemistry
DOI
10.3906/kim-1011-805
Abstract
A method was developed for the simultaneous determination of indole-3-acetic acid (IAA) and kinetin, based on the oxidation of both phytohormones at a pencil-lead graphite electrode. The electrochemical behaviors were examined by cyclic, linear sweep, and square-wave voltammetry in the pH range of 2-12. In Britton-Robinson buffer solution (pH 6), the oxidation peaks of IAA and kinetin are well separated by square-wave voltammetry. By adding an anionic surfactant (sodium dodecylsulfate) in a concentration of 50 \mu M, the sensitivity of kinetin was increased significantly without reducing the resolution. The detection limits were 0.14 and 0.11 \mu M (24.53 and 23.67 ng mL^{-1}) for IAA and kinetin, respectively. Applicability to assays of maize plant extracts was illustrated.
Keywords
Phytohormones, indole-3-acetic acid, kinetin, pencil-lead graphite electrode, square-wave voltammetry, anionic surfactant, maize seed
First Page
413
Last Page
426
Recommended Citation
YARDIM, YAVUZ and ŞENTÜRK, ZÜHRE
(2011)
"Voltammetric behavior of indole-3-acetic acid and kinetin at pencil-lead graphite electrode and their simultaneous determination in the presence of anionic surfactant,"
Turkish Journal of Chemistry: Vol. 35:
No.
3, Article 6.
https://doi.org/10.3906/kim-1011-805
Available at:
https://journals.tubitak.gov.tr/chem/vol35/iss3/6