Polarographic determination of anticancer drug vitamin K_3 with direct and indirect methods/application to serum and K_3 injection solution

Authors

ÜMMİHAN TAŞKOPARAN YILMAZ
GÜLER SOMER

DOI

10.3906/kim-1003-409

Abstract

In this work, direct and indirect methods for determination of vitamin K_3 were developed using differential pulse polarography. The reaction between sulfite and the quinone (Q) form of vitamin K_3 was used for indirect determination, since the sulfite peak at -0.7 V is sharp and very reproducible in 0.1 M HAc NaAc (pH = 5.5). It was found that at pH 4.5-5.5, this reaction was quantitative and very fast when the temperature was 45 °C. For its direct determination, on the other hand, vitamin K_3 was standardized by the indirect method using standard sulfite as the reducing agent. The calibration graph for vitamin K_3 (in Q form), using the peak at -1.0 V in a HAc-NaAc medium with a pH of 5.5, was linear for concentrations ranging from 5 \times 10^{-7} to 3 \times 10^{-5} M, and the limit of detection (LOD) was 1.5 \times 10^{-7} M. The proposed methods were successfully applied to the determination of vitamin K_3 in a clinical injection solution and in blood serum.

Keywords

Vitamin K_3, sulfite, direct, indirect, differential pulse polarography

First Page

201

Last Page

213

Recommended Citation

YILMAZ, ÜMMİHAN TAŞKOPARAN and SOMER, GÜLER (2011) "Polarographic determination of anticancer drug vitamin K_3 with direct and indirect methods/application to serum and K_3 injection solution," Turkish Journal of Chemistry: Vol. 35: No. 2, Article 4. https://doi.org/10.3906/kim-1003-409
Available at: https://journals.tubitak.gov.tr/chem/vol35/iss2/4