Separation of Abscisic Acid, Indole-3-Acetic Acid, Gibberellic Acid in 99 R (Vitis berlandieri x Vitis rupestris) and Rose Oil (Rosa damascena Mill.) by Reversed Phase Liquid Chromatography

Authors

MUSTAFA KELEN
EBRU ÇUBUK DEMİRALAY
SONGÜL ŞEN
GÜLEREN ÖZKAN ALSANCAK

DOI

-

Abstract

Plant hormones, specialized chemical substances produced by plants, are the main internal factors controlling growth and development. In this study the pH and polarity of the mobile phase were taken into consideration to optimize the mobile phase for the chromatographic separation of 3 important plant hormones: abscisic acid (ABA), indole-3-acetic acid (IAA) and gibberellic acid (GA_3). pK_a values of ABA, IAA and GA_3 were determined using retention factors. These 3 hormones were extracted from 99 R (Vitis berlandieri x Vitis rupestris) and rose oil (Rosa damascena Mill.) and the chromatographic method developed was used for the separation of these hormones.

Keywords

Abscisic acid, gibberellic acid, indole-3-acetic acid, plant hormones, resolution, RP-HPLC

First Page

603

Last Page

610

Recommended Citation

KELEN, MUSTAFA; DEMİRALAY, EBRU ÇUBUK; ŞEN, SONGÜL; and ALSANCAK, GÜLEREN ÖZKAN (2004) "Separation of Abscisic Acid, Indole-3-Acetic Acid, Gibberellic Acid in 99 R (Vitis berlandieri x Vitis rupestris) and Rose Oil (Rosa damascena Mill.) by Reversed Phase Liquid Chromatography," Turkish Journal of Chemistry: Vol. 28: No. 5, Article 8. Available at: https://journals.tubitak.gov.tr/chem/vol28/iss5/8