The in Vitro Effects of Dexamethasone on Sheep Lens Glucose-6-Phosphate Dehydrogenase

Authors

ŞÜKRÜ BEYDEMİR
DESTAN NİL KULAÇOĞLU
MEHMET ÇİFTÇİ
ÖMER İRFAN KÜFREVİOĞLU

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8\% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I_{50} value of the dexamethasone was 3.05 mM.

DOI

-

Keywords

Sheep eye lens, dexamethasone, glucose-6-phosphate dehydrogenase

First Page

601

Last Page

608

Recommended Citation

BEYDEMİR, Ş, KULAÇOĞLU, D. N, ÇİFTÇİ, M, & KÜFREVİOĞLU, Ö. İ (2003). The in Vitro Effects of Dexamethasone on Sheep Lens Glucose-6-Phosphate Dehydrogenase. Turkish Journal of Chemistry 27 (5): 601-608. https://doi.org/-