Turkish Journal of Chemistry
Abstract
An electrochemical biosensor for the voltammetric detection of DNA sequences related to the bloom-forming genera of cyanobacteria, Microcystis spp., is described. A specific DNA ``probe'' is designed based on sequence polymorphism within the 16S ribosomal DNA (rDNA) of the Microcystis spp. These single-stranded probes were immobilized onto carbon paste electrode (CPE) with the adsorption at a controlled potential. The probes were hybridized with the complementary ``target'' sequences at the electrode. The formed hybrids on the electrode surface were evaluated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) using methylene blue (MB) and tris (2,2' -- bipyridine) ruthenium (II) chloride ([Ru(bpy)_{3}]^{2+}) as the labels of hybridization. The response of the probe modified CPE to the exposure of the non--complementary oligonucleotide proves the specificity of the hybridization with the target. The two-bases mismatch could also be discriminated and specific detection of Microcystis spp. was achieved by using the difference between the voltammetric peaks of MB and [Ru(bpy)_{3}]^{2+} obtained with the probe and hybrid-modified CPEs. The detection of Microcystis spp. target DNA from real tap water and river water samples was also achieved.
DOI
-
Keywords
DNA Biosensor, Microcystis spp., Methylene Blue, Ruthenium Bipyridine.
First Page
851
Last Page
862
Recommended Citation
ERDEM, ARZUM; KERMAN, KAGAN; MERİÇ, BURCU; ÖZKAN, DİLŞAT; KARA, PINAR; and ÖZSÖZ, MEHMET (2002) "DNA Biosensor for Microcystis spp. Sequence Detection by Using Methylene Blue and Ruthenium Complex as Electrochemical Hybridization Labels," Turkish Journal of Chemistry: Vol. 26: No. 6, Article 7. Available at: https://journals.tubitak.gov.tr/chem/vol26/iss6/7