Turkish Journal of Chemistry
DOI
-
Abstract
Trypsin was immobilized to alumina by adsorption from its aqueous solutions. The activity of trypsin was increased by immobilization for peptide synthesis. With these immobilized enzymes Bz-Arg-Leu-NH_{2} dipeptide synthesis was carried out. Iso amylalcohol, tetrahydrofuran and acetonitrile were used as solvents giving the highest yield in the peptide formation reaction. The yield of the peptide was strongly dependent on water content in the reaction medium. The stability of alumina-immobilized trypsin is described.
Keywords
Peptide synthesis, trypsin, immobilization, organic solvent, alumina.
First Page
529
Last Page
534
Recommended Citation
YEŞİLOĞLU, YEŞİM and SAĞIROĞLU, AYTEN (2002) "Trypsin-Catalyzed Peptide Synthesis in Acetonitrile with Low Water Content," Turkish Journal of Chemistry: Vol. 26: No. 4, Article 9. Available at: https://journals.tubitak.gov.tr/chem/vol26/iss4/9
