Turkish Journal of Chemistry
DOI
-
Abstract
A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 \mu gml^{-1} of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.
Keywords
Dyes, derivative spectrophotometry, pharmaceutical products, Sunset Yellow, Quinoline Yellow
First Page
501
Last Page
508
Recommended Citation
ÖZGÜR, MAHMURE ÜSTÜN and KOYUNCU, İKBAL (2002) "The Simultaneous Determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in Syrups and Tablets by Second Derivative Spectrophotometry," Turkish Journal of Chemistry: Vol. 26: No. 4, Article 6. Available at: https://journals.tubitak.gov.tr/chem/vol26/iss4/6
