Incorporation of EDTA for the Elimination of Metal Inhibitory Effects in an Amperometric Biosensor Based on Mushroom Tissue Polyphenol Oxidase

Authors

ARZUM ERDEM
DİLŞAT ÖZKAN
BURCU MERİÇ
KAĞAN KERMAN
MEHMET ÖZSÖZ

DOI

-

Abstract

Enzyme based biosensors are highly selective devices which rely on the specific binding of the target analyte (the substrate) to the active-site regions of the enzyme. The enzymatic reaction between mushroom tissue polyphenol oxidase and its substrate phenol is coupled with the use of potassium ferrocyanide (K_{4}Fe(CN)_{6}) as the mediator in ammonia buffer solution (pH 8.80). The response of devices is often affected by the presence of inhibitors, which combine with the free enzyme in a manner that prevents substrate binding. The objective of this study is to describe an efficient and yet simple strategy to eliminate the interference of metals in amperometric biosensing. The methods relies on in situ removal of the interfering metal by a surface-bound complexing agent, disodium ethylenediaminetetraacetate (EDTA). EDTA is incorporated into the electrode by mixing it within the enzyme-containing carbon paste matrix. Mixed enzyme/carbon paste electrodes are receiving considerable attention for the preparation of fast responding biosensors.

First Page

231

Last Page

239

Recommended Citation

ERDEM, ARZUM; ÖZKAN, DİLŞAT; MERİÇ, BURCU; KERMAN, KAĞAN; and ÖZSÖZ, MEHMET (2001) "Incorporation of EDTA for the Elimination of Metal Inhibitory Effects in an Amperometric Biosensor Based on Mushroom Tissue Polyphenol Oxidase," Turkish Journal of Chemistry: Vol. 25: No. 2, Article 14. Available at: https://journals.tubitak.gov.tr/chem/vol25/iss2/14