Turkish Journal of Chemistry
Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC
A high-performance liquid chromatographic procedure is presented for the determination of hydrochlorothiazide (HCT) and enalapril maleate (EM) in pharmaceutical tablets. An aliquot of the sample is dissolved in 15% acetonitrile (ACN) containing thiophylline as an internal standard and chromatographed on a Supelcosil LC-8 (5 \mu m), (150 mm x 4.6 mm i.d.) column. The mobile phase was 3.0 mM tetrabutylammoniumhydrogen sulfate (TBAHS) in ACN/water/triethylamine (TEA), (14, 85.6, 6.4 V/V) adjusted to pH 4.1 by glacial acetic acid. The detection was at 220 nm. The method was tested for linearity, accuracy, recovery, and specificity.
HPLC, reverse phase, hydrochlorothiazide, enalapril maleate, stability, analysis
AL-MOMANİ, IDREES F. (2001) "Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC," Turkish Journal of Chemistry: Vol. 25: No. 1, Article 6. Available at: https://journals.tubitak.gov.tr/chem/vol25/iss1/6