Specific Determination of Hydrogen Peroxide With A Catalase Biosensor Based on Mercury Thin Film Electrodes

Authors

F. NİL ERTAŞ
SUNA TİMUR
EROL AKYILMAZ
ERHAN DİNÇKAYA

DOI

-

Abstract

A biosensor for specific determination of hydrogen peroxide was developed using catalase (EC 1.11.1.6) in combination with a mercury film electrode. Catalase was immobilized with gelatine by means of glutaraldehyde on the electrode surface. The biosensor response was monitored by following the reduction peak of dissolved oxygen at around -0.24 V. The peak current changes upon addition of hydrogen peroxide and gives a linear response in a concentration range of 1-50 x 10^{-6} M within a response time of 3 minutes.

First Page

95

Last Page

100

Recommended Citation

ERTAŞ, F. NİL; TİMUR, SUNA; AKYILMAZ, EROL; and DİNÇKAYA, ERHAN (2000) "Specific Determination of Hydrogen Peroxide With A Catalase Biosensor Based on Mercury Thin Film Electrodes," Turkish Journal of Chemistry: Vol. 24: No. 1, Article 11. Available at: https://journals.tubitak.gov.tr/chem/vol24/iss1/11