Turkish Journal of Chemistry
Article Title
DOI
-
Abstract
Polyphenoloxidase(PPO) of dog-rose fruit was extracted and purified through (NH\4)\2SO\4 precipitation and dialysis. The optimum conditions for this purpose, i.e., pH and temperature, were determined with 4-methylcatechol optimum pH and temperature and found to be pH 8.5 and 20\ci, respectively. The inhibition effect of ascorbic acid was investigated and the I_{50} value was found to be 1.57\times10^{-4} M. The activity of dog-rose PPO was investigated by electron paramagnetic resonance (EPR) at different pH levels and temperatures. The optimum conditions obtained from both spectrophotometry and EPR were compared.
First Page
188
Last Page
194
Recommended Citation
NUHOĞLU, Çiğdem; UCUN, Fatih; AYYILDIZ, Enise; YOĞURTÇU, Y. Kemal; ŞAKİROĞLU, Halis; and KÜFREVİOĞLU, İrfan (1997) "EPR Spectroscopy of Dog-Rose Polyphenoloxidase," Turkish Journal of Chemistry: Vol. 21: No. 3, Article 6. Available at: https://journals.tubitak.gov.tr/chem/vol21/iss3/6
