EPR Spectroscopy of Dog-Rose Polyphenoloxidase

Authors

Çiğdem NUHOĞLU
Fatih UCUN
Enise AYYILDIZ
Y. Kemal YOĞURTÇU
Halis ŞAKİROĞLU
İrfan KÜFREVİOĞLU

Abstract

Polyphenoloxidase(PPO) of dog-rose fruit was extracted and purified through (NH\4)\2SO\4 precipitation and dialysis. The optimum conditions for this purpose, i.e., pH and temperature, were determined with 4-methylcatechol optimum pH and temperature and found to be pH 8.5 and 20\ci, respectively. The inhibition effect of ascorbic acid was investigated and the I_{50} value was found to be 1.57\times10^{-4} M. The activity of dog-rose PPO was investigated by electron paramagnetic resonance (EPR) at different pH levels and temperatures. The optimum conditions obtained from both spectrophotometry and EPR were compared.

DOI

-

First Page

188

Last Page

194

Recommended Citation

NUHOĞLU, Ç, UCUN, F, AYYILDIZ, E, YOĞURTÇU, Y. K, ŞAKİROĞLU, H, & KÜFREVİOĞLU, İ (1997). EPR Spectroscopy of Dog-Rose Polyphenoloxidase. Turkish Journal of Chemistry 21 (3): 188-194. https://doi.org/-