EPR Spectroscopy of Dog-Rose Polyphenoloxidase

Authors

Çiğdem NUHOĞLU
Fatih UCUN
Enise AYYILDIZ
Y. Kemal YOĞURTÇU
Halis ŞAKİROĞLU
İrfan KÜFREVİOĞLU

DOI

-

Abstract

Polyphenoloxidase(PPO) of dog-rose fruit was extracted and purified through (NH\4)\2SO\4 precipitation and dialysis. The optimum conditions for this purpose, i.e., pH and temperature, were determined with 4-methylcatechol optimum pH and temperature and found to be pH 8.5 and 20\ci, respectively. The inhibition effect of ascorbic acid was investigated and the I_{50} value was found to be 1.57\times10^{-4} M. The activity of dog-rose PPO was investigated by electron paramagnetic resonance (EPR) at different pH levels and temperatures. The optimum conditions obtained from both spectrophotometry and EPR were compared.

First Page

188

Last Page

194

Recommended Citation

NUHOĞLU, Çiğdem; UCUN, Fatih; AYYILDIZ, Enise; YOĞURTÇU, Y. Kemal; ŞAKİROĞLU, Halis; and KÜFREVİOĞLU, İrfan (1997) "EPR Spectroscopy of Dog-Rose Polyphenoloxidase," Turkish Journal of Chemistry: Vol. 21: No. 3, Article 6. Available at: https://journals.tubitak.gov.tr/chem/vol21/iss3/6