The therapeutic potential of Salvia aegyptiaca extracts in ethanol-induced gastric ulcer:insights into macroscopic, histopathological, and biochemical mechanisms

Authors

WALID MAMACHEFollow
SMAIN AMIRAFollow
FATIMA BENCHIKHFollow
HASSIBA BENABDALLAHFollow
AMOR BENCHEIKHFollow
HIND AMIRAFollow
ROUMAISSA OUNISFollow
MOHAMMED ABDALLAH TORKIFollow
CHAHRAZED KAOUDOUNEFollow

Abstract

Background/aim: This study explores the antiulcer activity of different doses of Salvia aegyptiaca (SAE) methanol (ME) and decoctedextracts (DE) on ethanol-induced gastric ulcers in rats.Materials and methods: Female Wistar rats weighing 180–200 g were divided into five groups: control, omeprazole (positive control),and extract-treated (100, 200, and 400 mg/kg). Ulcers were induced with absolute ethanol 30 min after treatment with the extracts.The experiment was followed by macroscopic and histopathological examination. In vitro tests were also conducted to assess lipidperoxidation, catalase activity, mucus content, glutathione, and protein levels.Results: The study found that 100% ethanol caused significant damage, including colour and mucus loss, petechiae, haemorrhages,and oedema. However, pretreatment with ME SAE or DE SAE at doses of all three levels reduced the ethanol-induced damage.Histopathological analysis revealed reduced signs of haemorrhagic lesions, infiltration, and oedema in rats treated with ME SAE or DESAE at doses of 100 or 200 mg/kg, whereas the 400 mg/kg dose provided complete protection. Comparable to the use of omeprazole,ingestion of DE SAE at doses of 100, 200, or 400 mg/kg demonstrated substantial protection against stomach ulcers produced byethanol, with a range of 76%–84%. Both SAE extracts induced a dose-dependent increase in glutathione levels, with DE SAE showing asignificant rise at 200 and 400 mg/kg.Conclusion: The SAE extracts demonstrated a significant decrease in gastric lipid peroxidation, outperforming the effect of omeprazole.

Author ORCID Identifier

WALID MAMACHE: 0000-0002-8567-5634

SMAIN AMIRA: 0000-0003-4457-3591

FATIMA BENCHIKH: 0000-0001-6863-8818

HASSIBA BENABDALLAH: 0000-0002-6686-2207

AMOR BENCHEIKH: 0000-0002-8300-938X

HIND AMIRA: 0000-0002-7557-3212

ROUMAISSA OUNIS: 0000-0003-4437-9748

MOHAMMED TORKI: 0009-0001-7508-2958

CHAHRAZED KAOUDOUNE: 0000-0001-8677-5488

DOI

10.55730/1300-0152.2722

Keywords

Salvia aegyptiaca, gastric ulcer, lipid peroxidation, antioxidant enzyme

First Page

40

Last Page

51

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

MAMACHE, WALID; AMIRA, SMAIN; BENCHIKH, FATIMA; BENABDALLAH, HASSIBA; BENCHEIKH, AMOR; AMIRA, HIND; OUNIS, ROUMAISSA; TORKI, MOHAMMED ABDALLAH; and KAOUDOUNE, CHAHRAZED (2025) "The therapeutic potential of Salvia aegyptiaca extracts in ethanol-induced gastric ulcer:insights into macroscopic, histopathological, and biochemical mechanisms," Turkish Journal of Biology: Vol. 49: No. 1, Article 4. https://doi.org/10.55730/1300-0152.2722
Available at: https://journals.tubitak.gov.tr/biology/vol49/iss1/4