Turkish Journal of Biology

Author ORCID Identifier

NAZ ÜNSAL: 0000-0002-2705-5306

POLEN KOÇAK DENİZCİ: 0000-0003-4493-1061

HAZAL YILMAZ: 0000-0002-0930-3783

MERVE YILDIRIM CANPOLAT: 0000-0001-5363-8161

FİKRETTİN ŞAHİN: 0000-0002-7208-8967




Small extracellular vesicles (SEVs) are known to have an impact on the physiological circumstances of the target cells, are a critical component of cell-to-cell communication and have been implicated in a variety of diseases. Although it has been proposed that edible plant-derived nanoparticles have effect on communication with mammalian cells, the influence of these nanoparticles on cancer cell development has yet to be explored. In order to characterize small extracellular vesicles obtained from garlic, specific SEV surface markers and antibodies and size detections were performed by using scanning electron microscopy and nanoparticle tracking analysis. Human hepatoma, Hep3B, human neuroblastoma SH-SY5Y, human pancreatic adenocarcinoma, Panc-1, human glioblastoma, U87 and prostate cancer, PC-3 and human umbilical vein endothelial HUVEC cell lines were treated with garlic SEVs to examine their anticancer properties. Annexin V/pI staining for apoptosis, mRNA and protein expression levels via RT-PCR and ELISA. Results indicated that garlic SEVs triggered apoptosis by activating the intrinsic pathway. Our findings support that SEVs produced from garlic may trigger apoptotic cell death in cancer cells while having no effect on healthy cells. As a result, it was discovered that plant SEVs had anti-cancer effects via activating caspase mediated apoptosis. In summary, plant derived extracellular vesicles from garlic, have a lot of potential applications in medicine, particularly in the treatment of cancer without harming healthy cells.


Plant derived extracellular vesicles, apoptosis, cancer, garlic (Allium Sativum)

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Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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