X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g., proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception, single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice described in this article can be applied to any biological sample for structural studies. Therefore, this article will be a valuable practical guide from sample preparation to data analysis.
X-ray crystallography, light source, structural biology, atomic resolution, drug repurposing, drug development, structural dynamics
ATALAY, NECATİ; AKCAN, ENVER KAMİL; GÜL, MEHMET; AYAN, ESRA; DESTAN, EBRU; KUZUCU, FATMA BETÜL ERTEM; TOKAY, NURETTİN; ÇAKILKAYA, BARIŞ; NERGİZ, ZELİŞ; USTA, GÖZDE KARAKADIOĞLU; KEPCEOĞLU, ABDULLAH; YAPICI, İLKİN; TOSUN, BİLGE; BALDIR, NİLÜFER; YILDIRIM, GÜNSELİ; JOHNSON, J AUSTIN; GÜVEN, ÖMÜR; SHAFIEI, ALALEH; ARSLAN, NAZLI EYLÜL; YILMAZ, MERVE; KULAKMAN, CAHİNE; PAYDOS, SEYİDE SEDA; ÇİNAL, SEYNEP SENA; ŞABANOĞLU, KARDELEN; PAZARÇEVİREN, AYŞEGÜL; YILMAZ, AYŞENUR; CANBAY, BAŞAK; AŞÇI, BENGİSU; KARTAL, ESRA; TAVLI, SERRA; ÇALISEKİ, MEHMET; GÖÇ, GÜNCE; MERMER, ARİF; YEŞİLAY, GAMZE; ALTUNTAŞ, SEVDE; TATEISHI, HIROSHI; OTSUKA, MASAMI; FUJITA, MIKAKO; TEKİN, ŞABAN; ÇİFTÇİ, HALİLİBRAHİM; DURDAĞI, SERDAR; DOĞANAY, GİZEM DİNLER; KARACA, EZGİ; TÜRKÖZ, BURCU KAPLAN; KABASAKAL, BURAK VELİ; KATI, AHMET; and DEMİRCİ, HASAN
"Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source "Turkish DeLight","
Turkish Journal of Biology: Vol. 47:
1, Article 2.
Available at: https://journals.tubitak.gov.tr/biology/vol47/iss1/2