Glyphosate is a widely used herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-encoding aroA gene in the shikimate pathway. The discovery and cloning of the aroA gene with high resistance is central to breeding a transgenic glyphosate-resistant plant. A novel aroAPantoea gene from Pantoea G-1 was previously isolated and cloned. The aroAPantoea enzyme was defined as a new class I EPSPS with glyphosate resistance. The aroAPantoea gene was introduced into tobacco through Agrobacterium-mediated transformation. The transgenic tobacco plants were confirmed by PCR, RT-PCR, and Southern blot. The analysis of glyphosate resistance also showed that the transgenic tobacco plants could survive at 15 mM glyphosate; the glyphosate resistance level of the transgenic plants is higher than the agricultural application level recommended by most manufacturers. Overall, this study shows that aroA Pantoea can be used as a candidate gene for the development of genetically modified crops.
aroA, glyphosate, Pantoea, tobacco, transformation
LIU, FENG and CAO, YUEPING
"Expression of a bacterial aroA gene confers tolerance to glyphosate in tobacco plants,"
Turkish Journal of Biology: Vol. 42:
2, Article 9.
Available at: https://journals.tubitak.gov.tr/biology/vol42/iss2/9