Turkish Journal of Biology
DOI
10.3906/biy-1712-56
Abstract
Glyphosate is a widely used herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-encoding aroA gene in the shikimate pathway. The discovery and cloning of the aroA gene with high resistance is central to breeding a transgenic glyphosate-resistant plant. A novel aroAPantoea gene from Pantoea G-1 was previously isolated and cloned. The aroAPantoea enzyme was defined as a new class I EPSPS with glyphosate resistance. The aroAPantoea gene was introduced into tobacco through Agrobacterium-mediated transformation. The transgenic tobacco plants were confirmed by PCR, RT-PCR, and Southern blot. The analysis of glyphosate resistance also showed that the transgenic tobacco plants could survive at 15 mM glyphosate; the glyphosate resistance level of the transgenic plants is higher than the agricultural application level recommended by most manufacturers. Overall, this study shows that aroA Pantoea can be used as a candidate gene for the development of genetically modified crops.
Keywords
aroA, glyphosate, Pantoea, tobacco, transformation
First Page
187
Last Page
194
Recommended Citation
LIU, FENG and CAO, YUEPING
(2018)
"Expression of a bacterial aroA gene confers tolerance to glyphosate in tobacco plants,"
Turkish Journal of Biology: Vol. 42:
No.
2, Article 9.
https://doi.org/10.3906/biy-1712-56
Available at:
https://journals.tubitak.gov.tr/biology/vol42/iss2/9
