Turkish Journal of Biology




The possibility of using crude laccase in the dechlorination of chlorine-based bleached kraft pulp was investigated. Culture supernatants of seven white-rot fungal strains and kraft-pulp samples taken from E1 (Alkaline Extraction-1), E2 (Alkaline Extraction-2) and D2 (Chlorinedioxide treatment) stages of chlorine-based bleaching processes were used as the laccase source and substrates respectively during the studies. The dechlorination activity of Trametes versicolor was found to be more than that of the other fungi examined. The addition of a laccase inducer, xylidine, into the culture medium of T. Versicolor led to an increase in dechlorination activity. In the experiment performed to find out the role of laccase activity in dechlorination reactions, a considerable reduction in the dissolved oxygen concentration due to laccase-dependent dechlorination activity was observed. Stepwise enzyme treatment was found to be necessary for increasing the yield of dechlorination.


Biodechlorination, Kraft pulp, Laccase, Trametes (Coriolus) versicolor.

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