Turkish Journal of Biology
Abstract
In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.
DOI
-
Keywords
Early wheat embryo development, Germin, Western blotting.
First Page
775
Last Page
782
Recommended Citation
ÇALIŞKAN, MAHMUT and CUMING, ANDREW C. (2000) "Temporal and Spatial Determination of GerminBiosynthesis in Wheat Tissues," Turkish Journal of Biology: Vol. 24: No. 4, Article 9. Available at: https://journals.tubitak.gov.tr/biology/vol24/iss4/9
