Turkish Journal of Biology
Abstract
Cells require protein synthesis to perform the adhesion function properly. In order to dis-cover the effect of endogenous protein synthesis on CHL cell adhesion; the adhesion strengths of cul-tured Chinese Hamster Lung (CHL) cells that were grown for 24 hours on the glass surface in the pres-ence of 1µgml -1 of cycloheximide or emetine or, both were measured quantitatively using a converg-ing laminar flow chamber. The inhibition of protein synthesis by above drug(s) reduced cell adhesion strength significantly. Hence critical shear stress of detachment of cell were 8.68±1.52 Nm -2 , 8.13±1.48Nm -2 , and 6.57±1.01Nm -2 , respectively. While that of the control cells was 14.40±1.20Nm -2 . Before subculturing CHL cells in the presence of these drug(s), pre-treatment of cells with the same protein synthesis inhibitor even further reduced cell adhesion strength. Thus with pre-treatment of cycloheximide, emetine, both emetine and cycloheximide critical shear stress of detachment of cells were 3.24±0.73Nm -2 , 2.51±0.41Nm -2 , and 2.18±0.47Nm -2 , respectively. These results indicate that cells requires protein synthesis to gain possible maximum adhesion strength.
DOI
-
Keywords
CHL cell, cell adhesion, protein synthesis, emetine, cycloheximide.
First Page
397
Last Page
406
Recommended Citation
YILDIRIM, Ali and WHISH, William JD (1998) "The Role of Protein Synthesis on the AdhesionStrength of CHL Cells," Turkish Journal of Biology: Vol. 22: No. 4, Article 3. Available at: https://journals.tubitak.gov.tr/biology/vol22/iss4/3