Transformation Efficiency of R-Plasmids which were Isolated from Hospital Waste-water Bacteria

Authors

Sadık DİNÇER
Burhan ARIKAN
Ömer ÇOLAK
Hatice GÜVENMEZ

DOI

-

Abstract

As is widely known, hospital waste waters play a very important role in the increase and transmission of resistance in bacteria. A total of 52 transformation experiments were carried out using the CaCl2 method with R-plasmids which were isolated from hospital waste-water bacteria with plasmid-coded resistance to third-generation cephalosporins. Transformations were realised in 10 of the experiments in which 3 antibiotics were detected (19.2%). In 4 of the experiments 2 antibiotics were detected (7.7%), and in 18 of the experiments 1 antibiotic (34.6%). No transformation was obtained 20 of the experiments (38.4%).

Keywords

Bacterial transformation, Plasmids, Antibiotic resistance

First Page

293

Last Page

301

Recommended Citation

DİNÇER, Sadık; ARIKAN, Burhan; ÇOLAK, Ömer; and GÜVENMEZ, Hatice (1996) "Transformation Efficiency of R-Plasmids which were Isolated from Hospital Waste-water Bacteria," Turkish Journal of Biology: Vol. 20: No. 3, Article 14. Available at: https://journals.tubitak.gov.tr/biology/vol20/iss3/14