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Turkish Journal of Biology

Transformation Efficiency of R-Plasmids which were Isolated from Hospital Waste-water Bacteria

Abstract

As is widely known, hospital waste waters play a very important role in the increase and transmission of resistance in bacteria. A total of 52 transformation experiments were carried out using the CaCl2 method with R-plasmids which were isolated from hospital waste-water bacteria with plasmid-coded resistance to third-generation cephalosporins. Transformations were realised in 10 of the experiments in which 3 antibiotics were detected (19.2%). In 4 of the experiments 2 antibiotics were detected (7.7%), and in 18 of the experiments 1 antibiotic (34.6%). No transformation was obtained 20 of the experiments (38.4%).

DOI

-

Keywords

Bacterial transformation, Plasmids, Antibiotic resistance

First Page

293

Last Page

301

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