Preparation of cholesterol-imprinted polymer for selective adsorption of cholesterol from gastrointestinal mimicking solution

Authors

VEYİS KARAKOÇ, Çankırı Karatekin Üniversitesi: Cankiri Karatekin UniversitesiFollow
EROL ERÇAĞFollow

Author ORCID Identifier

0000-0002-2511-6478,0000-0003-4927-2405

DOI

10.55730/1300-0527.3667

Abstract

The purpose of this study is to synthesize a highly selective adsorbent to remove cholesterol, one of the most important causes of cardiovascular diseases, from the intestinal mimic solution (IMS). For this purpose, cholesterol imprinted polymers were synthesized by suspension polymerization method using the molecular imprinting technique. In the first step, the functional monomer MATyr with hydrophobic character was synthesized. Then, the cholesterol-MATyr monomer precomplex was formed and the polymerization process was carried out by adding cross-linkers with the comonomer HEMA. The synthesized polymer poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) poly(HEMA–MATyr) was characterized by FTIR and SEM. The cholesterol adsorbing behavior of the synthesized poly(HEMA–MATyr) microbeads adsorbent was investigated at different initial concentrations, different temperatures, and adsorption times. The maximum adsorption capacity of microbeads was determined as 56.67 mg/g at a concentration of 2.5 mg/L. The amount of cholesterol adsorbed in the IMS was found as 83.07 mg/g polymer, which indicates that 92% of the cholesterol in the medium was adsorbed. The selectivity behavior of the cholesterol imprinted polymer was carried out with the stigmasterol and estradiol molecules, which are similar in structure, molecular weight, and character to the cholesterol molecule. The chol-imprinted polymeric beads were 21.38 and 10.08 fold more selective for cholesterol compared to estradiol and stigmasterol steroids used as competitor agents respectively. Kinetic and isotherm calculations of the synthesized cholesterol imprinted polymer were made and reusability experiments were carried out.

Keywords

Molecularly imprinted polymers (MIP), intestinal mimicking solution(IMS), cholesterol removal, pseudospecific ligands, methacryloamidotyrosine (MATyr)

First Page

387

Last Page

401

Recommended Citation

KARAKOÇ, VEYİS and ERÇAĞ, EROL (2024) "Preparation of cholesterol-imprinted polymer for selective adsorption of cholesterol from gastrointestinal mimicking solution," Turkish Journal of Chemistry: Vol. 48: No. 2, Article 16. https://doi.org/10.55730/1300-0527.3667
Available at: https://journals.tubitak.gov.tr/chem/vol48/iss2/16