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Turkish Journal of Botany

Abstract

Viruses that naturally infect fungi capable of forming mycorrhizal symbioses remain poorly studied. In the present study, a new partitivirus associated with the basidiomycete mycorrhizal fungus Lactarius mediterraneensis was identified and characterized, and it was designated as Lactarius mediterraneensis partitivirus 1 (LmPV1). The complete genome of LmPV1 was determined through high throughput sequencing of double-stranded RNA (dsRNA) extracts and confirmed by Sanger sequencing of RLM-RACE PCR amplicons. T he genome comprises two dsRNA segments, 1899 bp and 1957 bp, each containing a single open reading frame (ORF) a putative RNA-dependent RNA polymerase (RdRp) and a capsid protein (CP). Comparative analysis using BLASTp revealed the closest matches to be Sarcosphaera coronaria partitivirus ANK VIR-52 (65.22% identity for RdRp) and Rosellinia necatrix partitivirus 26 (26.53% identity for CP). Phylogenetic reconstruction based on the RdRp sequence positioned LmPV1 within a cluster exclusively composed of Alphapartitivirus species from the Partitiviridae family. Given these molecular data, LmPV1 is proposed as a novel member of the Alphapartitivirus genus. This work provides the first documented evidence of a partitivirus infecting the mycorrhizal basidiomycete L. mediterraneensis.

Author ORCID Identifier

AIGUL KALİYEVA: 0009-0006-5718-253X

ERGİN ŞAHİN: 0000-0003-1711-738X

GÜLCE EDİŞ: 0000-0001-7038-9865

ILGAZ AKATA: 0000-0002-1731-1302

DOI

10.55730/1300-008X.2888

Keywords

Mycovirus, Partitivirus, Viral diversity, Lactarius sp.

First Page

103

Last Page

109

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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