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Turkish Journal of Botany

Author ORCID Identifier

GÖKHAN GÖKDEMİR: 0000-0001-9909-3662

Zafer SEÇGIN: 0000-0002-1952-1784

KARAM MOSTAFA: 0000-0003-2286-0434

MOHAMED ABDULLA: 0000-0001-6454-1410

MUSA KAVAS: 0000-0001-5903-2873

DOI

10.55730/1300-008X.2831

Abstract

Global climate change exposes crops to numerous abiotic and biotic stress factors, significantly threatening their growth, development, and overall productivity. Among the various abiotic stresses, salt and drought are the most detrimental, having a severe impact on yield stability. Late Embryogenesis Abundant proteins (LEA) represent a broad and varied class of polypeptides that accumulate in response to cellular dehydration across diverse organisms. Nonetheless, there has been a scarcity of studies on this protein family in common beans prior to this investigation. In this study, we identified 69 members of the LEA gene family in common bean, characterized by conserved LEA and dehydrin domains and additional functional domains. Based on their systematic evolutionary relationships, they were classified into eight subfamilies (LEA_1, LEA_2, LEA_3, LEA_4, LEA_5, LEA_6, DEHYDRIN, and SMP). In this study, approximately 62% of the identified PvLEAs are classified within the LEA_2 group, characterized by their higher hydrophobicity. Notably, they are clustered on chromosomes 7 and 8, suggesting their evolutionary conservation within these chromosomal regions. Analysis of promoter regions uncovered a variety of elements, including those responsive to stress, including ARE, ABRA, WRKY, TCA-element, and CGTCA-motif, implying their potential role in stress response mechanisms. The PvHDN3 overexpressed transgenic lines exhibited enhanced physiological health, increased height, and better-developed roots and leaves compared to the wild-type plants. In addition, the study found that PvHDN3 expression in response to different abiotic stress (salt and drought) intensity was closely linked to several stress-related physiological parameters, including proline and malondialdehyde (MDA) concentrations, ascorbate peroxidase (APX), catalase (CAT), and peroxidase (POX) activity. The current study offers novel insights into the formation of LEA proteins in common beans and their roles in developmental processes under stress conditions, providing a valuable foundation for future research.

Keywords

common bean, Dehydrin, Drought stress, Late embryogenesis abundant proteins (LEA), slat stress

First Page

502

Last Page

520

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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