Chemical fingerprints and bioactivities of 12 Anatolian Achillea species by LC-MS/MS with chemometric approach: Novel phytonutrients, natural food preservatives and chlorogenic acid sources

Authors

MUSTAFA ABDULLAH YILMAZ
ABDULSELAM ERTAŞ
İSMAİL YENER
FATMA PINAR TÜRKMENOĞLU
ÖZGE TOKUL ÖLMEZ
MEHMET ÖZTÜRK
MUHAMMED ALTUN
OĞUZ ÇAKIR
ABBAS TARHAN
MEHMET BOĞA
İBRAHİM DEMİRTAŞ
MEHMET HAKKI ALMA
HAMDİ TEMEL

DOI

10.55730/1300-008X.2723

Abstract

The objective of this study was to evaluate the biological activities and chemical fingerprint profiles of the extracts obtained from twelve Achillea L. species (A. lycaonica, A. biebersteinii (syn: A. arabica), A. kotschyi subsp. kotschyi, A. schischkinii, A. millefolium subsp. millefolium, A. sintenisii, A. setacea, A. teretifolia, A. wilhelmsii subsp. wilhelmsii (syn: A. santolinoides subsp. wilhelmsii), A. nobilis, A. goniocephala, A. spinulifolia). The antioxidant, enzyme inhibitory and cytotoxic effects were evaluated to investigate their bioactivity profiles. Furthermore, the total flavonoid and phenolic contents were determined and LC-MS/MS analysis was performed to reveal the phytochemical profile of the investigated extracts. A. kotschyi and A. nobilis species were detected to have very high antioxidant potential as well as high total phenolic content (260.00 ± 3.38 and 282.97 ± 3.14 ?g of PEs mg extract?1, respectively). According to the LC-MS/ MS results, A. kotschyi and A. nobilis species were found to contain very high concentrations of chlorogenic acid (55812.20 and 46407 ?g analyte g extract?1). Besides, the bioactivities and phenolic composition of these species were chemometrically analyzed using principal component analysis (PCA) and hierarchical clustering analysis (HCA) techniques. It has also been determined that Achillea species generally exhibit quite high cytotoxic activity against the HeLa cell line. The studied species showed high urease enzyme activities.

Keywords

Achillea, antioxidant, cytotoxicity, urease and tyrosinase, LC-MS/MS, chemometric analysis

First Page

473

Last Page

489

Recommended Citation

YILMAZ, MUSTAFA ABDULLAH; ERTAŞ, ABDULSELAM; YENER, İSMAİL; TÜRKMENOĞLU, FATMA PINAR; ÖLMEZ, ÖZGE TOKUL; ÖZTÜRK, MEHMET; ALTUN, MUHAMMED; ÇAKIR, OĞUZ; TARHAN, ABBAS; BOĞA, MEHMET; DEMİRTAŞ, İBRAHİM; ALMA, MEHMET HAKKI; and TEMEL, HAMDİ (2022) "Chemical fingerprints and bioactivities of 12 Anatolian Achillea species by LC-MS/MS with chemometric approach: Novel phytonutrients, natural food preservatives and chlorogenic acid sources," Turkish Journal of Botany: Vol. 46: No. 5, Article 5. https://doi.org/10.55730/1300-008X.2723
Available at: https://journals.tubitak.gov.tr/botany/vol46/iss5/5