Turkish Journal of Botany
DOI
-
Abstract
Electrophoretic analysis of germinating linseed proteins showed a gradual decrease in the quantity of a protein with a molecular weight of 42 kDa. This protein accumulates after 36 h of germination in synchronisation with an increase in lipase activity, and a decrease in the quantity of the total lipids. The 42 kDa subunit was found to be a lipid body membrane protein. This protein was isolated and identified by immunoprecipitation as a subunit of lipase. The linseed lipase acted on a wide range of triacylglycerols and had optimal activity at pH 4.7. The activity of the enzyme was slightly affected by a high concentration of salts and EDTA, while high concentrations of non-ionic detergents exhibited a pronounced inhibitory effect. These data suggest that the isolated 42 kDa protein is most likely a linseed acid lipase responsible for the breakdown of lipids during germination.
Keywords
Acid lipase, Triton X-100-solubilised lipid body membrane protein (XLBP), ether-extracted lipid body membrane protein (ELBP)
First Page
177
Last Page
184
Recommended Citation
SAMMOUR, REDA HELMY AHMED (2005) "Purification and Partial Characterisation of an Acid Lipase in Germinating Lipidbody Linseedlings," Turkish Journal of Botany: Vol. 29: No. 3, Article 2. Available at: https://journals.tubitak.gov.tr/botany/vol29/iss3/2
