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Turkish Journal of Botany

DOI

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Abstract

In vitro cultures of five moss species were established on hormone-free MS medium, or on MS medium supplemented with different concentrations of 2,4-dichlorophenoxyacetic acid and kinetin. In vitro culture of Eurhynchium praelongum (Hedw.) B., S. & G., has been initiated from the apical shoots of the gametophytes, and the cultures of Aloina aloides (K.F.Schultz) Kindb. Brachythecium velutinum (Hedw.) B., S. & G., Ceratodon purpureus (Hedw.) Brid. and Grimmia pulvinata (Hedw.) Sm. were initiated from the spores of immature sporophytes. In secondary protonema culture of Eurhynchium praelongum, spontaneous regeneration occurred successfully. Protonema cultures of Aloina aloides, Brachythecium velutinum and Ceratodon purpureus reversed to caulonema culture where bud formation occurred. Grimmia pulvinata cultures remained at the protonema stage.

Keywords

mosses, in vitro culture, Aloina aloides, Brachythecium velutinum, Ceratodon purpureus, Eurhynchium praelongum, Grimmia pulvinata

First Page

441

Last Page

446

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