Turkish Journal of Botany
DOI
-
Abstract
Somatic embryogenesis was initiated from immature zygotic embryos of Abies cilicica Carr. and its hybrid A. cilicica x A. nordmanniana. Schenk and Hildebrandt medium (SH) supplemented with 5 µM benzylaminopurine was used as the initiation medium. In A. cilicica, the initiation of embryonal suspensor mass (ESM) frequency ranged from 5.4 to 63.5%, and 28.6% of these cell lines formed mature somatic embryos. In A. cilicica x A. nordmanniana, from 3 to 27.6% of zygotic embryos formed ESM, and maturation of somatic embryos was observed in 34.8% lines. For somatic embryo maturation, Murashige, Skoog and SH media supplemented with 4% maltose and 10% polyethylene glycol-4000 were used. For maturation, 80 µM abscisic acid was most effective. After three weeks of partial desiccation, mature embryos germinated on SH medium with 1% sucrose and 1% activated charcoal, and plantlets with cotyledons, hypocotyls and radicles were obtained.
Keywords
Conifers, maturation, germination, plantlet regeneration
First Page
71
Last Page
76
Recommended Citation
VOOKOVA, BOZENA and KORMUTAK, ANDREJ (2003) "Plantlet Regeneration in Abies cilicica Carr. and Abies cilicica x Abies nordmanniana Hybrid via Somatic Embryogenesis," Turkish Journal of Botany: Vol. 27: No. 2, Article 1. Available at: https://journals.tubitak.gov.tr/botany/vol27/iss2/1
