Regeneration and Agrobacterium-mediated TransformationStudies in Tomato ( Lycopersicon esculentum Miller)

Authors

HÜSEYİN AVNİ ÖKTEM
YETER BÜLBÜL
EMRE ÖKTEM
MERAL YÜCEL

DOI

-

Abstract

An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtain rooted plants from leaf pieces of two tomato cul-tivars (ES58 and WC156) within 3-4 months. Here, we also present our preliminary results on transformation experiments carried out with 8-day-old cotyledon explants and Agrobacterium tumefaciensEHA105 strain.

Keywords

Tomato, regeneration, Agrobacterium, transformation, Lycopersicon

First Page

345

Last Page

348

Recommended Citation

ÖKTEM, HÜSEYİN AVNİ; BÜLBÜL, YETER; ÖKTEM, EMRE; and YÜCEL, MERAL (1999) "Regeneration and Agrobacterium-mediated TransformationStudies in Tomato ( Lycopersicon esculentum Miller)," Turkish Journal of Botany: Vol. 23: No. 5, Article 7. Available at: https://journals.tubitak.gov.tr/botany/vol23/iss5/7