Turkish Journal of Botany
DOI
-
Abstract
A method for in vitro micropropagation of sainfoin was developed. Following optimization of the growth regulator combinations in the media, large numbers of shoots were propagated from a single embryonic axis within 8 weeks on Murashige and Skoog (MS) media supplemented with combination of different 6-benzylaminopurine (BAP), indole-butyric acid (IBA) and ?-naphthaleneacetic acid (NAA) concentrations. The highest number of shoot multiplication was obtained on the media containing 2 mg/l BAP and either 0.05 or 0.1 mg/l IBA, and on the media supplemented with 2 mg/l BAP and either 0.05, 0.1 or 0.5 mg/l NAA, or 8 mg/l BAP and 0.05 mg/l NAA. The highest shoot length was observed on a medium containing 2 mg/l BAP only. Excised shootsrooted at a frequency of 60 % within 4 weeks in half strength MS medium containing 1 mg/l IBA and grew into normal fertile plants.
Keywords
Sainfoin, Onobrychis viciifolia, micropropagation, shoot multiplication, shoot tip culture
First Page
133
Last Page
136
Recommended Citation
SANCAK, CENGİZ (1999) "In Vitro Micropropagation of Sainfoin ( Onobrychis viciifolia Scop.)," Turkish Journal of Botany: Vol. 23: No. 2, Article 8. Available at: https://journals.tubitak.gov.tr/botany/vol23/iss2/8
