Biological evaluation of novel 6,9-disubstituted purine analogues in high-grade serous ovarian cancer cell lines

Authors

DUYGU ALTIPARMAKFollow
DEREN DEMİREL YAVUZFollow
PINAR KUL KARADENİZLİFollow
İREM DURMAZ ŞAHİNFollow
MERAL TUNÇBİLEKFollow

Abstract

Background/aim: High-grade serous ovarian cancer (HGSOC) remains one of the most aggressive forms of ovarian malignancy and frequently shows resistance to conventional therapies. This study aimed to synthesize a novel series of purine analogues, 6-[(4-substituted benzyl amine)/(4-substituted aniline)]-9-cyclopentyl purines, and evaluate their anticancer efficacy against HGSOC cell lines.

Materials and methods: We assessed the biological effects of the synthesized purine analogues on the OVCAR3, OVSAHO, and KURAMOCHI HGSOC cell lines using the sulforhodamine B assay. To investigate the mechanism of action, we conducted flow cytometry and western blot analyses, focusing on DNA replication and apoptosis.

Results: Among the tested compounds, compound 8 showed significant cytotoxic activity with IC50 values in the low micromolar range. Preliminary data from flow cytometry and western blot analyses indicated that compound 8 may inhibit DNA replication and induce apoptosis, as reflected by changes in cell viability and cell-cycle progression.

Conclusion: Compound 8 may disrupt key proliferative mechanisms in cancer cells by interfering with DNA synthesis and activating programmed cell death pathways. These findings suggest that compound 8 is a promising lead candidate for further development in ovarian cancer therapeutics.

Author ORCID Identifier

DUYGU ALTIPARMAK: 0009-0003-4130-8597

DEREN DEMİREL YAVUZ: 0009-0000-4705-9294

PINAR KUL KARADENİZLİ: 0000-0003-3639-0496

İREM DURMAZ ŞAHİN: 0000-0001-5037-7883

MERAL TUNÇBİLEK: 0000-0002-7410-0202

DOI

10.55730/1300-0152.2788

Keywords

Cytotoxicity, high-grade serous ovarian cancer, apoptosis, cell-cycle arrest, synthesis, purine analogues

First Page

29

Last Page

36

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

ALTIPARMAK, D, DEMİREL YAVUZ, D, KUL KARADENİZLİ, P, DURMAZ ŞAHİN, İ, & TUNÇBİLEK, M (2026). Biological evaluation of novel 6,9-disubstituted purine analogues in high-grade serous ovarian cancer cell lines. Turkish Journal of Biology 50 (1): 29-36. https://doi.org/10.55730/1300-0152.2788