•  
  •  
 

Turkish Journal of Biology

Abstract

Previous studies on GTF2E showed that it has association with certain group of diseases such as colon cancer and trichothiodystrophy but what is the global effect of GTF2E on cellular processes is not widely characterized. In this regard, this study aimed to investigate and characterize the affect of GTF2E on transcription level of genes and identify the cellular processes and diseases associated with GTF2E. The HCT116 cell line was used in the study and transfected at 30 nM concentration with siGTF2E1 or non-target negative siRNA. After 72 hours, cells were harvested and prepared for further analysis. Whole transcriptome analysis was performed on the human colorectal carcinoma (HCT116) cell line obtained from siGTF2E1 knockdown HCT116 cells (n=3) and their non-target negative siRNA controls (n=3) using RNA-seq. We tested cell viability using MTS assay. Compared with the control group, 166 genes were identified at the time of GTF2E1 knockdown and expressed differentially in the knockdown group, including 66 up-regulated genes and 100 down-regulated genes. One significantly enriched GO term was identified, involving carbohydrate binding. One oncogene related to B-CLL called BCL11A was identified. Five genes associated with colon carcinoma were determined. Eleven genes involved in the development of atherosclerosis were identified.GTF2E1 knockdown caused a decrease in cell viability. In conclusion, the GTF2E1 knockdown group exhibited an altered expression of multiple genes and some of those genes are related to the development of atherosclerosis, colon carcinoma, and B cell chronic lymphocytic leukemia (B-CLL), which might shed light on the different regulatory affect of GTF2E and its association with certain diseases.

Author ORCID Identifier

SERDAR BAYSAL: 0000-0003-3394-2005

DOI

10.55730/1300-0152.2718

Keywords

TFIIE, RNA sequencing, transcription, atherosclerosis, colon cancer, B-CLL

First Page

442

Last Page

455

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Included in

Biology Commons

Share

COinS